When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

In a recent study posted to the medRxiv* preprint server, researchers examined sample collection practices, epidemiological characteristics, and cycle threshold (Ct) values for polymerase chain ...

This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR ...

In the summer of 1984, Henry Erlich, Glenn Horn, Randy Saiki, and myself were working on developing a DNA test for sickle cell anemia (SCA) at Cetus Corp. SCA is an autosomal dominant disease caused ...

Polymerase chain reaction (PCR) test kits for COVID-19 use what’s known as gene cloning – not reproductive cloning – to detect the presence or absence of the SARS-CoV-2 virus, but social media posts ...